Journal: The World Journal of Men's Health
Article Title: Human Adipose Stem Cells Engineered to Express Carboxylesterase Confer Anti-Tumoral Efficacy of Irinotecan in Castration-Resistant Prostate Cancer Bone Metastasis Growth and Osteolysis
doi: 10.5534/wjmh.240284
Figure Lengend Snippet: Migration of CE-expressing ADSC to prostate cancer cell cultures, potentially mediated by chemoattraction. (A, B) Cell migration assay using Matrigel™-coated Transwell chambers. hTERT-ADSC, hTERT-ADSC-CE1, and hTERT-ADSC-CE2 cells were plated in the upper chambers and co-cultured with empty media, WPMY-1 (WPM, human prostate myofibroblast cell line) or PC3 (human prostate cancer cell line) in the bottom chambers, as indicated. Migrated cells were stained, photographed (A) and quantified (B). (C–E) Expression of chemoattractant factors such as vascular endothelial growth factor (VEGF), stem cell factor (SCF) and stromal cell-derived factor 1 (SDC-1) and the corresponding receptors c-KIT, chemokine receptor 4 (CXCR4), VEGF receptors (VEGFR)-1, -2 and -3. Values are presented as mean±standard error of the mean. (A, B) ** p<0.01 compared with the ADSC/Media group. ## p<0.01 compared with the ADSC/WPMY-1 group. $$ p<0.01 compared with the ADSC/PC3 group. AA p<0.01 compared with the ADSC.CE1/PC3 group. BB p<0.01 compared with the ADSC.CE2/PC3 group. (C–E) * p<0.05 and ** p<0.01 compared with PC3. # p<0.05 and ## p<0.01 compared with ADSC, $$ p<0.01 compared with ADSC.CE1.CE: carboxylesterase, ADSC: adipose-derived stem cells, hTERT: human telomere reverse transcriptase gene, CPT-11: irinotecan.
Article Snippet: Medium or 1.5×10 4 WPMY-1, a human prostate myofibroblast cell line (ATCC), were loaded in the lower well of the 24-well plates 24 hours before the experiment started.
Techniques: Migration, Expressing, Cell Migration Assay, Cell Culture, Staining, Derivative Assay, Reverse Transcription